Paper-based microfluidic devices are attractive, low-cost and user-friendly diagnostic platforms for nucleic acids (NAs) amplification and testing. However, they have limited clinical applications due to their relatively lower sensitivity and the substantial amount of NAs as required for detection. Therefore, sensitive and specific detection of the NAs using paper-based microfluidics requires incorporation and developing on the same device of sample preparations steps and amplification. The project proposes to design, fabricate and test a sample-to-answer 3D modular-microfluidic point of care platform (HEAD-NAs) that aim to work at with low NAs concentrations. The platform integrates four different modules: filtration, lysis, preconcentration and amplification. The original elements are related to: the method for sample preconcentration, the electrochemical lysing module, the thermophoretic convective valve, the design structure for NA amplification. A testing kit for detection enteric pathogens, having 5 independent detection points (one for control) for simultaneous detection of: Enteropathogenic/Enterohemorrhag ic E. coli, Campylobacter spp., Salmonella spp., rotavirus will be developed. The HEAD-NAs platform presents a great potential for commercialisation further national and EU collaborations.