- Development of bio-sensing applications based on micro-optical components
Chemo/biosensors based on Surface plasmon Resonance (SPR) have been experimented using polimeric microprisms obtained by replication of an original (glass prism). The prism base is covered by a gold layer and a senzitive layer- fig. 11.
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Fig. 11 Sensor structure
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a) b)
Fig. 12.a) Images of the replicated prisms (with and without Au on the base);
b) image of the characteriation set-up.
The structures were used for alchool detection (fig. 13) and anti-colagen detection (fig. 14). In the second case a sensitive layer with colagene molecules have been used.
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Fig. 13. Sensor response (chemical sensing) function on analyte refractive index.
The refractive index depends on the concentration.
Experimental dots are for an alcool concentration of 5, 10, 20 and 25%.
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Fig. 14. Sesor response (biosensing of anti-colagene) function on no. of anticolagene droplets deposed on the surface.
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