• Development of  bio-sensing applications based on micro-optical components

  Chemo/biosensors based on Surface plasmon Resonance (SPR) have been experimented using polimeric microprisms obtained by replication of an  original (glass prism). The prism base is covered by a gold layer and a senzitive layer- fig. 11.

  
  Fig. 11 Sensor structure

   
  a)                                                        b)

  Fig. 12.a) Images of the replicated prisms (with and without Au on the base);
  b) image of the characteriation set-up.

  The structures were used for alchool detection (fig. 13)  and anti-colagen detection (fig. 14). In the second case a sensitive layer with colagene molecules have been used.
  

Fig. 13. Sensor response (chemical sensing) function on analyte refractive index.
The refractive index depends on the concentration. 
Experimental dots are for an alcool concentration of  5, 10, 20 and 25%.

Fig. 14. Sesor response (biosensing of anti-colagene) function on no. of anticolagene droplets deposed on the surface.