The need for an efficient catalysis and regeneration process involving the nicotinamide adenine dinucleotide is justified, by its economical weight as well as by its valuable applications in the biotechnology field. Nanostructured materials exhibit interesting properties which favour the electrochemical detection of NADH. Obstacles for the effective use of nicotinamide adenine dinucleotide include the need of high overpotentials for direct oxidation or reduction of the cofactor, electrode fouling, dimerization of the cofactor, etc. Nevertheless, to promote economically efficient processes, the regeneration of the pyridine cofactor remains a key problem to solve. A platform for various dehydrogenase based bioassays should be obtained by developing an electrochemical probe based on layer-by-layer self-assembled doped metallic nanoparticles membranes. When using nanoparticles for catalysis two main issues are raising: the stabilization the particles while retaining sufficient catalytic activity and the problematic separation of the catalytic particles from the reaction product and unused reactants at the end of the reaction. One solution may be the immobilization of the nanoparticles in thin membranes, minimizing the mass transfer limitations. A generic platform offering a fast regeneration and an efficient catalysis of coenzyme is the goal of this proposal. The motivation, the goals, the team and the methodology are fully described in this research proposal.
