The general objective of the project is to develop a nanostructured detection platform onto which a solid-phase isothermal amplification will be implemented for the rapid detection of bacteria/fungal DNA.

The specific objectives of the project are:

1) the design and testing of primer sets and the optimization of LAMP or RPA reactions;

2) the development of a nanostructured Si platform with an appropriate surface functionalization for the binding of capture probes;

3) the manufacturing of Polydimethylsiloxane (PDMS) microcavities that will act as reaction chambers for the isothermal amplification;

4) the statistical analysis of the data, and a parallel detection by PCR, in order to prove the functionality of the developed xPOC platform.