Project description

The aim of this project is to make some biochips using Microarray Technology (TMA) for allergies diagnosing and in the same time, to fabricate of a Platform for electro/optical characterization of the Biohibridic Interfaces (PIB). The research area is a cutting edge one, and is absolutely new in Romania.

This project is a combination between the fundamental and applicative research, at theoretical and biomedical applications level.
The result of research work involved in this project will consist in: 1. a biochip for quick diagnosis of the allergies by specific immunoglobulins (Ig E) immobilisation on technological micro fabricated surfaces; and 2) a PIB platform for the electrochemical reactions investigation to the interface between the substrate and biological material.

The advantages of this chip are: the increase of the analyzed data number in real time; a good specificity and sensibility of the results and the absence of the false positive and false negative reactions; allergies quick diagnosis. The allergens immobilisation by covalent bonding on a biochip surface is faster and more stable and it needs a smaller incubation time. 

The bio-chips will be designed and made in such way to allow the simultaneous analysis of a great number of allergen proteins on a small surface, giving the possibility to identify IgEs specifics by a unique determination using small serum volumes (nano-grams).

The development and fabrication of the bio-chip, and also, of a new method for quick and precisely diagnosing of the allergens, will have an important contribution in the field of bio-nano-systems and proteomics.